Effect of chelating agents on phytoxicity and bioaccumulation of heavy metals in vascular plants

Authors

  • Miroslav Horník Department of Biotechnology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
  • Jana Guldanová Department of Biotechnology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
  • Martin Pipíška Department of Biotechnology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
  • Jana Marešová Department of Biotechnology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
  • Jozef Augustín Department of Biotechnology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic

DOI:

https://doi.org/10.36547/nbc.1136

Keywords:

cadmium, cobalt, bioaccumulation, phytotoxicity, Nicotiana tabacum, speciation

Abstract

In this work the effect of chelating agents ethylenediaminetetraacetate (EDTA) and nitrilotriacetate (NTA) on phytotoxicity and bioaccumulation of Cd and Co in tobacco plants (Nicotiana tabacum L.) hydroponically grown in diluted Hoagland media (HM) spiked with 109Cd and 60Co was studied. Speciation analysis using a program Visual MINTEQ showed, that the portion of bioavailable ionic Me2+ forms significantly decreased in the presence of EDTA or NTA in 25% HM for account of [Me-EDTA]2- or [Me-NTA]- complexes. We found that the equimolar addition of EDTA or NTA to 50 μmol/dm3 CdCl2 or CoCl2 in HM positively diminished phytotoxicity of Cd or Co on tobacco plants. Bioaccumulation of Cd by tobacco roots during 8 d cultivation was minimally affected in the presence of equimolar concentrations of EDTA or NTA to 10 μmol/dm3 CdCl2 in media. On the contrary, equimolar concentration of EDTA or NTA added into HM caused considerable decrease of Co uptake by tobacco roots. Cadmium showed higher mobility in conductive tissues of tobacco plants than cobalt and the transport ratio in the presence of EDTA or NTA increased 2-times or 3-times in comparison with control experiments (without addition of chelates), respectively. In the case of cobalt this effect was observed in a less extent. Obtained data suggest the possibilities and constraints in the use of chelating agents in phytoextraction technologies in term of phytotoxicity, uptake and translocation of metals in plant tissues.

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Published

2021-09-02

How to Cite

Horník, M., Guldanová, J., Pipíška, M., Marešová, J., & Augustín, J. (2021). Effect of chelating agents on phytoxicity and bioaccumulation of heavy metals in vascular plants. Nova Biotechnologica Et Chimica, 9(3), 271-278. https://doi.org/10.36547/nbc.1136

Issue

Section

Research Articles
Received 2021-09-02
Published 2021-09-02