Low abundant bovine colostrum proteins in combination with amaranth oil reveal topical analgesic activity

Authors

  • Nail J. Sagdiev Institute of Bioorganic Chemistry of Uzbekistan Academy of Sciences, 100125, 83, M. Ulughbek Str., Tashkent, Uzbekistan
  • Jamolitdin F. Ziyavitdinov Institute of Bioorganic Chemistry of Uzbekistan Academy of Sciences, 100125, 83, M. Ulughbek Str., Tashkent, Uzbekistan
  • Nodir Sh. Berdiev Institute of Bioorganic Chemistry of Uzbekistan Academy of Sciences, 100125, 83, M. Ulughbek Str., Tashkent, Uzbekistan
  • Soyib S. Bozorov Institute of Bioorganic Chemistry of Uzbekistan Academy of Sciences, 100125, 83, M. Ulughbek Str., Tashkent, Uzbekistan
  • Tohir A. Khudoyberdiev Institute of Bioorganic Chemistry of Uzbekistan Academy of Sciences, 100125, 83, M. Ulughbek Str., Tashkent, Uzbekistan
  • Shukhratjon S. Olimjonov Institute of Bioorganic Chemistry of Uzbekistan Academy of Sciences, 100125, 83, M. Ulughbek Str., Tashkent, Uzbekistan
  • Natalya L. Vypova Institute of Bioorganic Chemistry of Uzbekistan Academy of Sciences, 100125, 83, M. Ulughbek Str., Tashkent, Uzbekistan
  • Akmal M. Asrorov Institute of Bioorganic Chemistry of Uzbekistan Academy of Sciences, 100125, 83, M. Ulughbek Str., Tashkent, Uzbekistan, Ajou University in Tashkent, 100204, 113A, Asalobod Str., Tashkent, Uzbekistan

DOI:

https://doi.org/10.36547/nbc.1246

Keywords:

Amarant oil, Analgesic activity, Bovine colostrum, Low-abundant proteins, Water-alcohol-soluble fraction

Abstract

Colostrum is an arsenal of proteins and peptides required at the earliest stage of a newborn development. Proteins involved in all biological processes of an infant development have been found in its composition. It is expected as cost-effective source of biologically active proteins and peptides. In this work, we separated water-alcohol-soluble low abundant proteins from bovine colostrum in preparative amounts that were further utilized in combination with amaranth oil for topical cream composition. The ratio of the obtained proteins’ fraction made a thousandth of the colostrum dry mass. The partial sequences of 37 identified proteins were established by mass-spectrometer and using BLAST search in NCBI database. In our previous work, we established the chemical composition of amaranth seed oil with ~6 % squalene by mass. The physical mixtures of these natural resources were fabricated into cream using hyaluronic acid as moisturizing agent and their analgesic activities were established. The optimal ratio of proteins and oil was determined in terms of their effects as analgesic means by experiments carried out on mice. Several proteins could possibly be responsible for the revealed biological efficacy. Among them, G-protein coupled receptor and synaptotagmin were previously linked with analgesic activity. Establishing an optimum ratio of ingredients proved also the contribution of higher quantity of amaranth oil, a rich source of squalene and unsaturated fatty acids.

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Published

2022-06-03

How to Cite

Sagdiev, N. J., Ziyavitdinov, J. F., Berdiev, N. S., Bozorov, S. S., Khudoyberdiev, T. A., Olimjonov, S. S., Vypova, N. L., & Asrorov, A. M. . (2022). Low abundant bovine colostrum proteins in combination with amaranth oil reveal topical analgesic activity. Nova Biotechnologica Et Chimica, 21(1), e1246. https://doi.org/10.36547/nbc.1246

Issue

Section

Research Articles
Received 2021-10-21
Accepted 2021-12-01
Published 2022-06-03