Enhanced vitamin B12 production using paneer whey as culture medium

Authors

  • Sangavi Gopalakrishnan Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, Tamilnadu, India
  • Harini Rajkumar Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, Tamilnadu, India
  • Usha Antony College of Fish Nutrition and Food Technology, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Chennai, Tamilnadu, India
  • Lakshmi B.S Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, Tamilnadu, India
  • Nandhini Devi Ganesan Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, Tamilnadu, India

DOI:

https://doi.org/10.34135/nbc.1339

Keywords:

Lactobacillus reuteri DSM 20016, Vitamin B12, Box Behnken design, Response surface methodology, Liquid paneer whey

Abstract

Vitamin B12, a water-soluble vitamin, plays a vital role in regulating metabolism in many organisms. However, its synthesis is restricted to limited bacteria and archaea. Therefore, industrial microbial fermentation is considered as a robust method to meet the demands of the vitamin B12. Lactobacillus reuteri DSM 20016 is one of the vitamin B12 producers. In the present study, liquid paneer whey is used as the medium along with the supplements, glycerol, and cobalt chloride to enhance the vitamin B12 production. The effect of liquid paneer whey, yeast extract, glycerol, and cobalt chloride on the vitamin B12 production was further optimized using Box Behnken design. Under optimized conditions, the production of the vitamin B12 reached up to 213.449 μg.L-1. The current study validated the feasibility of utilizing liquid paneer whey as a medium to produce high yield vitamin B12 by Lactobacillus reuteri DSM 20016.

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Published

2023-02-03

How to Cite

Gopalakrishnan, S., Rajkumar, H., Antony, U., B.S, L., & Ganesan, N. D. (2023). Enhanced vitamin B12 production using paneer whey as culture medium. Nova Biotechnologica Et Chimica, 22(1), e1339. https://doi.org/10.34135/nbc.1339

Issue

Vol. 22 No. 1 (2023)

Section

Research Articles

License

Copyright (c) 2023 Sangavi Gopalakrishnan, Harini Rajkumar, Usha Antony, Lakshmi B.S, Nandhini Devi Ganesan

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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