Elicitation Phenolic Compounds in Cell Culture of Vitis vinifera L. by Phaeomoniella chlamydospora

Authors

  • MARTIN SÁK
  • IVANA DOKUPILOVÁ
  • DANIEL MIHÁLIK
  • JANA LAKATOŠOVÁ
  • MARCELA GUBIŠOVÁ
  • JÁN KRAIC

DOI:

https://doi.org/10.1515/nbec-2015-0006

Keywords:

Vitis vinifera L., Phaeomoniella chlamydospora, phenolics, cell culture, elicitation

Abstract

The in vitro cell cultures of Vitis vinifera L. cv. St. Laurent were treated with two elicitors - synthetic methyl jasmonate and natural, prepared from grapevine plant infected with the Phaeomoniella chlamydospora, the agent causing the Esca disease of grapevine. Efficiency of phenolic compounds production after elicitation of cell culture was analysed immediately after treatment (15 min, 30 min, 60 min) and later (after 24, 48, and 72 hours). The cell growth and content of phenolic compounds (+)-catechin, (-)-epicatechin, p-coumaric acid, syringaldehyde, rutin, vanillic acid, and trans-resveratrol were analysed in cultivated cells as well as in cultivation medium. Pch-treatment increased production of total polyphenols the most significantly 15 min after the elicitation and in optimal time was 2.86 times higher than in nonelicited culture and 1.44 times higher than in MeJa induced cell culture.

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How to Cite

SÁK, M. ., DOKUPILOVÁ, . I. ., MIHÁLIK, D. ., LAKATOŠOVÁ, J. ., GUBIŠOVÁ, M. ., & KRAIC, J. . (2014). Elicitation Phenolic Compounds in Cell Culture of Vitis vinifera L. by Phaeomoniella chlamydospora. Nova Biotechnologica Et Chimica, 13(2), 162–171. https://doi.org/10.1515/nbec-2015-0006

Issue

Vol. 13 No. 2 (2014)

Section

Research Articles

License

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