Separation Of Methionine Enantiomers By Using Teicoplanin And Cyclofructan Columns

Authors

  • KATARÍNA HROBOŇOVÁ
  • ZUZANA DEÁKOVÁ
  • JAKUB MORAVČÍK
  • JOZEF LEHOTAY
  • DANIEL W. ARMSTRONG
  • ANNA LOMENOVÁ

DOI:

https://doi.org/10.1515/nbec-2015-0009

Keywords:

methionine, enantiomers, cyclofructan 6-based column, teicoplanin-based column, mobile phase composition, HPLC

Abstract

Methionine is a naturally occurring amino acid. Its enantiomeric separation by using high performance liquid chromatography on various types of chiral stationary phases was studied. The effect of mobile phase composition on enantioselectivity and retention was considered. The separation of the enantiomers was attained in different separation modes – reversed phase mode for the macrocyclic antibiotic chiral stationary phases (teicoplanin, teicoplanin aglycone), normal phase and polar organic phase modes for the isopropyl carbamate cyclofructan 6 chiral stationary phase. It was shown that the hydrogen bonding, dipole interactions, steric effects between methionine molecules and stationary phases play an important role in the separation of enantiomers.

Downloads

Published

2015-07-24

How to Cite

HROBOŇOVÁ, K. ., DEÁKOVÁ, Z. ., MORAVČÍK, J. ., LEHOTAY, . J. ., W. ARMSTRONG, D. ., & LOMENOVÁ, A. . (2015). Separation Of Methionine Enantiomers By Using Teicoplanin And Cyclofructan Columns. Nova Biotechnologica Et Chimica, 14(1), 1–11. https://doi.org/10.1515/nbec-2015-0009

Issue

Vol. 14 No. 1 (2015)

Section

Research Articles

License

All papers published in the Nova Biotechnologica et Chimica (NBC)  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).