HPLC Enantioseparation of Phenylcarbamic Acid Derivatives by Using Macrocyclic Chiral Stationary Phases

Authors

  • KATARÍNA HROBOŇOVÁ
  • JOZEF LEHOTAY
  • JOZEF ČIŽMÁRIK

DOI:

https://doi.org/10.1515/nbec-2016-0002

Keywords:

HPLC, chiral stationary phase, structure of the analyte, esters of phenylcarbamic acid

Abstract

The HPLC by using chiral stationary phases based on macrocyclic antibiotics, dimethylphenyl carbamate cyklofructan 7 and β-cyclodextrin in terms of polar-organic separation mode (mobile phase methanol/acetonitrile/acetic acid/triethylamine) were used for enantioseparation of alkoxy derivatives of phenylcarbamic acid. The effect of the analyte structures on the efficiency of enantioseparation was investigated. The most suitable stationary phase was teicoplanin aglycone, where the separations of the enantiomers were obtained (the resolution value from 0.65 to 2.90, depending on the structure of the analyte). Significant effect on the resolution of the enantiomers has position of alkoxy substituent in the hydrophobic part of the molecule. The enantiorecognition was achieved for 3-alkoxysubstituted derivatives.

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How to Cite

HROBOŇOVÁ, K. ., LEHOTAY, J. ., & ČIŽMÁRIK, J. . (2016). HPLC Enantioseparation of Phenylcarbamic Acid Derivatives by Using Macrocyclic Chiral Stationary Phases. Nova Biotechnologica Et Chimica, 15(1), 12–22. https://doi.org/10.1515/nbec-2016-0002

Issue

Vol. 15 No. 1 (2016)

Section

Research Articles

License

All papers published in the Nova Biotechnologica et Chimica (NBC)  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).