Sequences analysis of chloroplast psbA-trnH region in Citrus L. (Rutaceae) species from the Aegean region of Turkey

Authors

  • Emre Sevindik Department of Agricultural Biotechnology, Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey
  • Gaye Zeynep Canbolat Department of Agricultural Biotechnology, Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey
  • İlayda İrem Moral Department of Agricultural Biotechnology, Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey
  • Monika Sujka Deptartment of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, 8 Skromna St., Lublin 20-704, Poland

DOI:

https://doi.org/10.36547/nbc.877

Keywords:

Citrus, cpDNA, psbA-trnH, sequence analysis

Abstract

In this study, sequences analysis of some Citrus species distributed in Turkey's Aegean region was based on the cpDNA psbA-trnH  region. The sequences for psbA-trnH regions of the outgroups were retrieved from NCBI GenBank. Genomic DNA was isolated from healthy and green leaves. Total genomic DNA was extracted using GeneMark DNA isolation Plant Kit. The psbA-trnH region was amplified using primers psbA and trnH. DNA sequences were edited using the Sequencher 5.4.6. Sequencing data were analyzed using MEGA 6.0 software. Maximum likelihood (ML) tree was created to determine the relationships between Citrus taxa.  cpDNA psbA-trnH  sequences ranged between 426 and 470 nucleotides. Maximum likelihood phylogenetic tree is composed of two clades. The divergence values differed between 0.000 and 0.012. According to the results of the study, the separation of Citrus species in phylogenetic tree obtained with psbA-trnH sequence data was realized. However, it has been found that cpDNA psbA-trnH sequence populations of species belong together. In addition, the phylogenetic relationship between the sequence data of some species belonging to the Rutaceae family taken from NCBI and Citrus species was revealed.

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Published

2021-11-30

How to Cite

Sevindik, E., Canbolat, G. Z., Moral, İlayda İrem, & Sujka, M. (2021). Sequences analysis of chloroplast psbA-trnH region in Citrus L. (Rutaceae) species from the Aegean region of Turkey. Nova Biotechnologica Et Chimica, 20(2), e877. https://doi.org/10.36547/nbc.877

Issue

Section

Research Articles
Received 2021-02-22
Accepted 2021-10-06
Published 2021-11-30