TY - JOUR AU - Berdiev, Nodir Sh. AU - Ziyavitdinov, Jamolitdin F. AU - Asrorov, Akmal M. AU - Olimjonov, Shukhratjon S. AU - Salikhov, Shavkat I. PY - 2019/08/17 Y2 - 2024/03/29 TI - Characterization of a novel lipase from Pseudomonas aeruginosa JF - Nova Biotechnologica et Chimica JA - Nova Biotechnol. Chim. VL - 18 IS - 1 SE - Research Articles DO - 10.2478/nbec-2019-0006 UR - https://journals.scicell.org/index.php/NBC/article/view/509 SP - 44–51 AB - <p>Lipases cleaving oils into fatty acids and glycerol are of great interest for the use in increasing the efficiency of fuels. In this work, a novel lipase from&nbsp;<em>Pseudomonas aeruginosa</em>,&nbsp;<em>P. aeruginosa</em>&nbsp;A12, was isolated by ion-exchange and hydrophobic chromatography. The purity of lipase was shown by electrophoresis and its molecular weight was estimated to be ~ 31.6 kDa. The whole amino acid sequence was analyzed by an LC-MS/MS method. Temperature- and pH-dependent optimum of the enzyme compiled 30 °C and 7.5, respectively. The obtained enzyme exhibited 79 % similarity of amino acid sequence to a lipase isolated from the same strain of&nbsp;<em>P. aeruginosa</em>. Thus, the novel lipase was determined to belong to I.1 subfamily of bacterial true lipases. Three dimensional structure of the isolated lipase isoform was modeled based on obtained sequences. Amino acids forming the catalytic domain were shown in the model. Lid domain is suggested to be in the open conformation. These results provide a potential alternative for enzymatic digestion of fuel oils and serve for the development of fundamental knowledge of lipase activity.</p> ER -