TY - JOUR AU - Enibukun, Jesupemi Mercy AU - Boboye, Bolatito Esther PY - 2020/06/30 Y2 - 2024/03/29 TI - Molecular characterization and evaluation of crude oil remediation potential of some rhizobia isolated from plant root nodules JF - Nova Biotechnologica et Chimica JA - Nova Biotechnol. Chim. VL - 19 IS - 1 SE - Research Articles DO - 10.36547/nbc.v19i1.580 UR - https://journals.scicell.org/index.php/NBC/article/view/580 SP - 80-88 AB - <p>This study aimed to determine the molecular identities and genetic relatedness of rhizobia isolated from pigeon pea and pinto beans, and assess their remediation potential in the presence of 1 %, 3 % and 5 % (w/v) crude oil in minimal medium for 7 days incubation period. Standard microbiological and molecular methods which include amplification and purification of 16S rRNA, agarose gel electrophoresis, and sequencing. Results showed molecular identities of six rhizobia from pigeon peas as <em>Bradyrhizobium</em> <em>diazoefficiens</em> USDA122, <em>Rhizobium</em><em> leguminosarum</em> WSM2304, <em>Bradyrhizobium</em><em> japonicum</em> N61, <em>Rhizobium</em><em> leguminosarum</em> N741, <em>Rhizobium</em><em> leguminosarum</em> BIHIB1217, and <em>Bradyrhizobium</em><em> japonicum</em> E109; and three rhizobia obtained from pinto beans were <em>Rhizobium</em> <em>leguminosarum</em> N871, <em>Bradyrhizobium</em><em> diazoefficiens</em> USDA110 and <em>Bradyrhizobium</em><em> japonicum</em> SEMIA5079. All tested rhizobia (9) showed petroleum degradation ability, as they all grew in the 1, 3 and 5 % (w/v) crude oil minimal medium under laboratory conditions. <em>B. diazoefficiens </em>USDA122 showed the highest optical density (OD) value of 1.184 ± 0.05 on 7th day at 1 % (w/v) crude oil contamination, while <em>R. leguminosarum</em> N741 has the lowest OD value of 0.372 ± 0.02 at 5 % (w/v) crude oil on 7<sup>th</sup> day. For all the rhizobia, increase occurred throughout incubation period at 1, 3 and 5 % (w/v) except <em>Rhizobium leguminosarum</em> N741 and <em>R. leguminosarum</em> BIHIB1217. In conclusion, the association of <em>R. leguminosarum</em> BIHIB1217 and <em>R. leguminosarum</em> N871 from pigeon pea and pinto beans respectively, were found most effective in crude oil degradation and thus they are recommended as a promising association for remediation of crude oil spilled soils.</p> ER -