TY - JOUR AU - Mourad, Guetouache AU - Toumatia, Omrane AU - Medjekal, Samir AU - Guessas, Bettache AU - Cheriet, Sarah AU - Bouras, Noureddine PY - 2022/07/15 Y2 - 2024/03/28 TI - Phenotypic, genotypic, and technological characterization of new lactic acid bacterium isolated from traditional dairy product in Djelfa, Algeria JF - Nova Biotechnologica et Chimica JA - Nova Biotechnol. Chim. VL - 21 IS - 2 SE - Research Articles DO - 10.36547/nbc.904 UR - https://journals.scicell.org/index.php/NBC/article/view/904 SP - e904 AB - <p>A total of 33 lactic acid bacteria (LAB) were isolated from 5 samples of traditional dairy product (Jben) collected from various livestock farms in Oued El Malha, Djelfa, the Ouled Naïl Range of north-central Algeria. The taxonomic study of the strain B04 using a multiphase approach based on morphology, physiology, molecular (16S rRNA) and phylogenetic analyses allowed correlating this strain to <em>Enterococcaceae</em>. The difference of the 16S rRNA gene sequence of the isolate toward the most closely related genus <em>Enterococcus</em> was more than 6 % suggesting that the strain B04 represents a new genus. The results of the evaluation of some physiological tests indicated that B04 exhibited good biological activities including acidifying, proteolytic and bacterial inhibition. The strain B04 was antagonistic toward <em>Staphylococcus aureus</em> ATCC 6538, <em>Escherichia coli</em> ATCC 25922, and <em>Bacillus cereus</em> ATCC 10876.</p> ER -