Isolation and statistical optimization of rhodanese (a thiosulphate sulphur transferase) production potential of Klebsiella oxytoca JCM 1665 using response surface methodology

Authors

  • Babamotemi Oluwasola Itakorode 1Department of Chemical Sciences, Oduduwa University Ipetumodu, Ile-Ife, Osun State, Nigeria, 2Department of Biochemistry and Molecular Biology, Faculty of Science, Obafemi Awolowo University Ile-Ife, Osun State, Nigeria
  • Raphael Emuebie Okonji Department of Biochemistry and Molecular Biology, Faculty of Science, Obafemi Awolowo University Ile-Ife, Osun State, Nigeria

DOI:

https://doi.org/10.36547/nbc.1083

Keywords:

Klebsiella oxytoca, Optimization, Polymerase chain reaction , Response surface methodology, Rhodanese

Abstract

Microorganisms are increasingly being used in cyanide bioremediation. Several organisms have been reported to thrive in cyanide contaminated wastewater due to their ability to produce cyanide detoxifying enzymes. However, to improve the production efficiency of these enzymes combinations of process variables need to be optimized. In this study, Klebsiella oxytoca JCM 1665 was isolated from industrial wastewater, identified by sequencing its 16S rRNA gene and subjected to rhodanese production using submerged fermentation. The conditions for production were optimized using response surface methodology (RSM). Central composite design was employed to evaluate the effects of three production parameters – peptone (1 – 5 %), KCN (0.1 – 0.5 %), and time of incubation (1 – 24 h). Second-order polynomial model was used to predict the response. Rhodanese activity in the experiments varied from 0.05 to 7.5 RU.mg-1.  Under the optimum conditions of 4.35 % peptone, 0.4 % KCN and incubation time of 13 hr., the value for rhodanese yield was 7.810 U.mL-1.  The R2 value for the model was 0.9925 (R2 = 0.9925). Also, the experimental values are in accordance with those predicted, indicating the suitability of the employed model and the success of RSM in optimizing the production conditions.

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Published

2022-06-03

How to Cite

Itakorode, B. O., & Okonji, R. E. (2022). Isolation and statistical optimization of rhodanese (a thiosulphate sulphur transferase) production potential of Klebsiella oxytoca JCM 1665 using response surface methodology. Nova Biotechnologica Et Chimica, 21(1), e1083. https://doi.org/10.36547/nbc.1083

Issue

Section

Research Articles
Received 2021-08-02
Accepted 2021-12-20
Published 2022-06-03