Cr and Ni simultaneous phytotoxicity and mutagenicity assay

Authors

  • Agáta Fargašová Department of Ecosozology and Physiotactics, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina, SK-842 15 Bratislava, Slovak Republic
  • Jana Lištiaková Department of Ecosozology and Physiotactics, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina, SK-842 15 Bratislava, Slovak Republic

DOI:

https://doi.org/10.36547/nbc.1156

Keywords:

chromium, nickel, Vicia sativa, phytotoxicity, chromosomal aberation assay, Tradescantia, micronucleus assay

Abstract

For genotoxicity study simultaneous phytotoxicity and mutagenicity assay with Vicia sativa L. var. Klára was used. For phytotoxicity the following rank orders of growth inhibition can be arranged: for roots: Ni(II) > Cr(VI) > Cr(III); for shoots: Ni(II) > Cr(VI) ≥ Cr (III). For mutagenicity assay root tips of V. sativa were used and chromosome aberrations were determined at least in 500-anatelophases. All tested metals exerted in V. sativa a significant increase of chromosomal aberration rate in applied concentrations. Maximum of aberrations invoked Cr(VI) and the rank order of aberrations fall was: Cr(VI) > Ni(II) > Cr(III). Genotoxic effects of metals were determined by analysis of micronuclei frequency in the pollen tetrads of Tradescantia plants. None of tested metal significantly stimulated micronuclei frequency and genotoxic effect was decreased in order: Cr(VI) ≥ Ni(II) > Cr(III).

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Published

2021-11-22

How to Cite

Fargašová, A., & Lištiaková, J. (2021). Cr and Ni simultaneous phytotoxicity and mutagenicity assay. Nova Biotechnologica Et Chimica, 9(2), 107-112. https://doi.org/10.36547/nbc.1156

Issue

Section

Research Articles
Received 2021-09-10
Published 2021-11-22