Microbial dechlorination of polychrolinated biphenyls

Authors

  • Vlasta Dudková Institute of Chemical Technology in Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Technická 3, 166 28 Prague 6; Czech Republic
  • Kateřina Demnerová Institute of Chemical Technology in Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Technická 3, 166 28 Prague 6; Czech Republic
  • Donna L. Bedard Rensselaer Polytechnic Institute, Department of Biology, 110 Eighth Street, Troy, NY 12180-3590, U.S.A.

DOI:

https://doi.org/10.36547/nbc.1158

Keywords:

polychlorinated biphenyls, reductive dechlorination, gas chromatography

Abstract

Polychlorinated biphenyls (PCBs) are organic xenobiotics contaminating environment for at least 50 years. They could be eventually eliminated by various organisms under different conditions. The degree of chlorine substitution per biphenyl molecule influences biodegradability which decreases with increasing chlorination. Our work is focused on the PCBs biodegradation under anaerobic conditions. The suitable high chlorinated biphenyls are converted via reductive dechlorination to the chlorinated biphenyls with lower extent of chlorine, which could be eventually fully mineralized by aerobic bacteria. Microbial consortium was isolated from sediment of Strážský Creek (located near by plant producing PCBs in the past). This consortium was able to dechlorinate polychlorinated biphenyls under anoxic conditions. The effectiveness of this process was tested under different cultivation condition – different energetic sources (Aroclor 1248 or Aroclor 1260 or Delor 103 or Delor 106), addition of potential electron donors (pyruvate, lactate or acetate with hydrogen) and further if there is necessary to add yeast extract into fresh low sulphur cultivation media. Our microbial consortia so far do not need supplementation by non-contaminated sediment to maintain dechlorination activity. Addition of yeast extract is non essential, but needs to be further proved in serial transfers. In all cases (except acetate without yeast extract) dechlorination proceeds at meta- and flanked paraposition.

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Published

2021-11-22

How to Cite

Dudková, V., Demnerová, K., & Bedard, D. L. (2021). Microbial dechlorination of polychrolinated biphenyls. Nova Biotechnologica Et Chimica, 9(2), 119-124. https://doi.org/10.36547/nbc.1158

Issue

Section

Research Articles
Received 2021-09-10
Published 2021-11-22