Optimization of rosmarinic acid extraction from lemon balm (Melissa officinalis)
Keywords:rosmarinic acid, optimization, Melissa officinalis, extraction
AbstractThe aim of this study was optimization of rosmarinic acid extraction from lemon balm (Melissa officinalis). The optimal conditions for the extraction of rosmarinic acid from lemon balm were determined using response surface methodology (RSM). A center composide design (CCD) was used to investigate the effects of three independent variables, namely solid-liquid radio, solvent composition (%) and extraction temperature (°C). Dependent variable was yield of rosmarinic acid. A second-order polynomial model was used for predicting the response. Optimized conditions for rosmarinic acid were: PEME 1:29 (w/v), temperature 66 °C a % propan-2-ol 34 %. The experimental values agreed with predicted within a 95 % confidence interval. Yield of rosmarinic acid extraction by these optimized conditions was achieved 72.6 mg of rosmarinic acid / g of dry extraction matter.
How to Cite
Ondrejovič, M., Benkovičová, H., & Šilhár, S. (2021). Optimization of rosmarinic acid extraction from lemon balm (Melissa officinalis). Nova Biotechnologica Et Chimica, 9(2), 175-182. https://doi.org/10.36547/nbc.1275
Copyright (c) 2021 Nova Biotechnologica et Chimica
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.