The possibility to enhance flavonoids production in Rubia tinctorum L. callus cultures

Authors

  • Daniela Kákoniová Institute of Chemistry Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38 Bratislava, Slovak Republic
  • Štefánia Vaverková Department of Botany and Pharmacognosy, Faculty of Pharmacy Comenius University, Kalinčiakova 8, SK-832 32 Bratislava, Slovak Republic
  • Desana Lišková Institute of Chemistry Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38 Bratislava, Slovak Republic
  • Eva Urgeová Faculty of Natural Sciences, University of SS. Cyril and Methodius, Trnava, Slovak Republic
  • Zuzana Juráková Institute of Chemistry Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38 Bratislava, Slovak Republic

DOI:

https://doi.org/10.36547/nbc.1277

Keywords:

cadmium, callus, growth, madder, secondary metabolites

Abstract

Production of flavonoids in madder callus culture (Rubia tinctorum L.) was dependent on culture conditions and culture media composition. The content of flavonoids increased in calli maintained on media supplemented with NAA (4 mg.l-1) or NAA:BAP (4 mg.l-1 a 1 mg.l-1) in 16 h photoperiod. Flavonoids represented 2.08 – 2.25 % of callus dry mass. The presence of Cd(NO3)2 (3.1 or 31.0 mg.l-1 concentrations) negatively influenced callus growth, but enhaced the percentage of dry mass in callus cells. During 42 days of culture an increase of cadmium accumulation and even of flavonoids has been observed. The most considerable influence of CdCl2 or Cd(NO3)2 on flavonoids content has been shown in short-term experiments after 48 h of callus culture. More distinct influence has been observed under the treatment with CdCl2 (0.005 mg.l-1) in comparison with Cd(NO3)2.

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Published

2021-11-22

How to Cite

Kákoniová, D. ., Vaverková, Štefánia ., Lišková, D. ., Urgeová, E. ., & Juráková, Z. . (2021). The possibility to enhance flavonoids production in Rubia tinctorum L. callus cultures. Nova Biotechnologica Et Chimica, 9(2), 191-197. https://doi.org/10.36547/nbc.1277

Issue

Section

Research Articles
Received 2021-11-22
Published 2021-11-22