Antibiofilm activities of crude extract and supernatant of Actinobacteria against biofilm formed by foodborne pathogens and food spoilage bacteria
Keywords:Actinobacteria, Anti-biofilm, Anti-quorum sensing, Chromobacterium violaceum, Food-borne pathogens
Bacteria form a biofilm that attaches the microbial population to a solid surface, thus acting as a barrier to protect external stresses from the bacterial community. In the food industry, biofilms are believed to be responsible for food-borne diseases and food spoilages. This research was conducted to characterize anti-quorum sensing (QS), anti-biofilm activity of crude extract, and supernatant from Actinobacteria isolates against biofilm of foodborne, and food spoilage bacteria. Actinobacteria is the group of filamentous spore-forming Gram-positive bacteria, which is well recognized as a source for novel secondary metabolites, such as anti-biofilm and anti-quorum sensing. Two isolates of Actinobacteria utilized in this study were recovered from the Indonesian marine environment in the previous study. Both isolates demonstrated anti-QS activity on early detection assay using the agar overlay method. The Actinobacteria isolates were fermented, and the crude extracts were obtained through extraction using 100 % ethyl acetate and further evaporation, while the supernatant was collected directly following fermentation. The entire Actinobacteria isolates displayed capability as an anti-biofilm agent in crude extracts form at a concentration of 20 mg.mL-1, alongside when in its supernatant form. The Actinobacteria isolates in both extracts and supernatants form demonstrated anti-QS activity based on the N-Hexanoyl-L- homoserine lactone (HHL) validation assay and were also categorized as non-toxic based on Brine Shrimp Lethality Assay (BSLA).
How to Cite
Copyright (c) 2023 Faustina Audrey Agatha, Diana Elizabeth Waturangi
This work is licensed under a Creative Commons Attribution 4.0 International License.
All papers published in the Nova Biotechnologica et Chimica (NBC) are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).