Multiplex PCR method for detection of variability in genes encoding the alpha proteins of Streptococcus agalactiae

Authors

  • Barbora Vidová Institute of Biochemistry, Nutrition and Health, Slovak University of Technology, SK-812 37 Bratislava, Slovak Republic
  • Michal Chotár Institute of Molecular Biology, Slovak Academy of Sciences SK-845 51, Bratislava, Slovak Republic
  • Jozef Timko Institute of Molecular Biology, Slovak Academy of Sciences SK-845 51, Bratislava, Slovak Republic
  • Andrej Godány Department of Biotechnology, University of SS. Cyril and Methodius, SK-917 01 Trnava, Slovak Republic

DOI:

https://doi.org/10.36547/nbc.1288

Keywords:

Streptococcus agalactiae, alpha-like family, variability, multiplex PCR

Abstract

The majority of Streptococcus agalactiae strains express one or more surface-anchored proteins that vary by strain. These proteins, which are characteristic for S. agalactiae, and are able to induce protective antibodies, include the alpha C, Rib proteins, alpha-like protein 2, and alpha-like protein 3. In this study was developed multiplex PCR method for detection of genes encoding these proteins, and its occurrence within a various S. agalactiae isolates of bovine origin. Also are reported two new genes from bovine isolates of S. agalactiae amplified by PCR, encoding other putative members of the family, alphalike protein 6, and alpha-like protein 7. They contain an overall genetic organization highly similar to that of the alpha C and Rib proteins.

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Published

2021-11-29

How to Cite

Vidová, B., Chotár, M., Timko, J., & Godány, A. (2021). Multiplex PCR method for detection of variability in genes encoding the alpha proteins of Streptococcus agalactiae. Nova Biotechnologica Et Chimica, 9(1), 63–72. https://doi.org/10.36547/nbc.1288

Issue

Vol. 9 No. 1 (2009)

Section

Research Articles

License

Copyright (c) 2021 Barbora Vidová, Michal Chotár, Jozef Timko, Andrej Godány

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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