DNA damage by oxidized fatty acids detected by DNA/SPE biosensor

Authors

  • Ľudmila Sirotová Food Research Institute, Department Biocentre, Kostolná 7, SK- 900 01 Modra, Slovak Republic
  • Marcela Matulová Food Research Institute, Department Biocentre, Kostolná 7, SK- 900 01 Modra, Slovak Republic

DOI:

https://doi.org/10.36547/nbc.1307

Keywords:

DNA/SPE biosensor, fatty acid oxidation, DNA damage

Abstract

Electrochemical DNA/screen-printed electrode biosensor (DNA/SPE biosensor) was tested for the detection of alterations in DNA formed as a consequence of the reaction between DNA and oxidative products of fatty acids. Interaction of DNA with a mixture of products generated during the oxidation of linoleic and oleic acids manifested DNA damage depending on a tested fatty acid and the presence of hydroperoxides and thiobarbituric acid reactive substances (TBARS) determined after the oxidation of fatty acids. A bigger extent of the DNA damage was registered in the case of the interaction with oxidized linoleic acid with the high content of TBARS. The results achieved suggest the possible application of DNA/SPE biosensor in the detection of an interaction between DNA and products of fatty acid oxidation.

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Published

2021-12-04

How to Cite

Sirotová, Ľudmila, & Matulová, M. (2021). DNA damage by oxidized fatty acids detected by DNA/SPE biosensor. Nova Biotechnologica Et Chimica, 8(1), 45–53. https://doi.org/10.36547/nbc.1307

Issue

Vol. 8 No. 1 (2008)

Section

Research Articles

License

Copyright (c) 2021 Ľudmila Sirotová, Marcela Matulová

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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