Bioaccumulation of 60Co2+ ions in tobacco plants

Authors

  • Ľuboš Vrtoch Department of Biotechnology, University of SS. Cyril and Methodius, SK-917 01 Trnava, Slovak Republic
  • Martin Pipíška Department of Biotechnology, University of SS. Cyril and Methodius, SK-917 01 Trnava, Slovak Republic
  • Miroslav Horník Department of Biotechnology, University of SS. Cyril and Methodius, SK-917 01 Trnava, Slovak Republic
  • Jozef Augustín Department of Biotechnology, University of SS. Cyril and Methodius, SK-917 01 Trnava, Slovak Republic

DOI:

https://doi.org/10.36547/nbc.1357

Keywords:

cobalt, 60Co2 , Nicotiana tabacum L., accumulation, hydroponics, autoradiography

Abstract

Tobacco has previously been used in investigations of metals and radionuclide uptake. This study presents determination of bioaccumulation and translocation of 60Co2+ ions in tobacco plants (Nicotiana tabacum L.) grown in Hoagland’s nutrient solution. Cobalt concentration in tobacco plants increased with increasing concentration in nutrient solution. Bioaccumulation from the initial concentration C0 = 0.96 μM Co reached 90% after 7 day cultivation. Only small amounts of Co accumulated in roots, up to 2 - 4 % were removable from roots by washing with 0.1 M CoCl2, indicating that this portion of Co is bound to the root surface in ion-exchangeable form. Tobacco roots retained approximately 2/3 of accumulated cobalt and 1/3 was transported to shoots. Autoradiography revealed that 60Co was preferentially localized in younger leaves. Prolongation of cultivation time did not change the [Co]roots : [Co]shoots ratio significantly. Relationships between growth rate, transpiration rate, uptake and distribution of cobalt in plant tissue are discussed.

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Published

2022-01-13

How to Cite

Vrtoch, Ľuboš, Pipíška, M., Horník, M., & Augustín, J. (2022). Bioaccumulation of 60Co2+ ions in tobacco plants. Nova Biotechnologica Et Chimica, 7(1), 41–49. https://doi.org/10.36547/nbc.1357

Issue

Vol. 7 No. 1 (2007)

Section

Research Articles

License

Copyright (c) 2022 Ľuboš Vrtoch, Martin Pipíška, Miroslav Horník, Jozef Augustín

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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