Improving PHB yield in Halomonas halophila through medium optimization

Authors

  • Zuzana Baťová Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, J. Herdu 2, Trnava, SK-91701, Slovak Republic
  • Ľubomíra Jurečková Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, J. Herdu 2, Trnava, SK-91701, Slovak Republic
  • Daniela Chmelová Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, J. Herdu 2, Trnava, SK-91701, Slovak Republic
  • Miroslav Ondrejovič Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, J. Herdu 2, Trnava, SK-91701, Slovak Republic

DOI:

https://doi.org/10.34135/nbc.2132

Keywords:

Polyhydroxyalkanoates, Halomonas halophila, Optimization, Basic culture medium, Biomass yield

Abstract

Polyhydroxyalkanoates (PHAs) offer a sustainable substitute for conventional petroleum-based plastics. Halophiles possess a remarkable ability to produce PHAs from low-cost by-products like crude whey or molasses, offering a sustainable alternative to traditional plastics. This study highlights the efficient production of PHAs by Halomonas halophila CCM 3662 using a basic culture medium designed to simultaneously enhance biomass and polyhydroxybutyrate (PHB) production. Through optimization via response surface methodology (RSM), the optimal medium composition was identified as 22.85 g/l glucose, 4.36 g/l ammonium sulfate, and 13.22 g/l sodium hydrogen. Under these conditions, biomass production reached 1.44±0.07 g/l, while PHB content achieved 50.16±0.84%. This represented a 2.8-fold increase in biomass production and a 2.5-fold increase in PHB production compared to a medium without optimized composition. This study provides valuable insights into the optimal medium composition for the growth of the halophilic H. halophila and its potential application in PHB production for various uses.

Downloads

Published

2024-12-29

How to Cite

Baťová, Z., Jurečková, Ľubomíra, Chmelová, D., & Ondrejovič, M. (2024). Improving PHB yield in Halomonas halophila through medium optimization. Nova Biotechnologica Et Chimica, 23(3), e2132. https://doi.org/10.34135/nbc.2132

Issue

Vol. 23 No. 3 (2024)

Section

Research Articles

License

Copyright (c) 2024 Zuzana Baťová, Ľubomíra Jurečková, Daniela Chmelová, Miroslav Ondrejovič

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

All papers published in the Nova Biotechnologica et Chimica (NBC)  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).