Actinomyces Ruminicola G10 - The Rumen Bacterium Recovered from Glycerol Enriched Cultivation Media

Authors

  • ANNA VANDŽUROVÁ
  • GABRIEL BÓDY
  • PETER JAVORSKÝ
  • PETER PRISTAŠ

DOI:

https://doi.org/10.2478/nbec-2013-0004

Keywords:

rumen bacteria, sheep, glycerol, Actinomyces ruminicola

Abstract

Gradual increasing of glycerol concentration up to 10% using sheep ruminal fluid as an inoculum for in vitro cultivation was accompanied by significant changes in bacterial population as documented by DGGE analysis. The resulting bacterial consortium was composed of three dominant bacteria with Actinomyces related bacterium to be predominant. Upon cultivation on media with glycerol as a sole carbon source a single bacterium was cultivated from this consortium. Isolate G10 was found to be anaerobic, Gram-positive rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequences showed that G10 isolate is related to the Actinomyces ruminicola species (97.7% of similarity). The role of rumen actinobacteria is largely unknown and their participation in glycerol utilization (tolerance) has not been described yet. The G10 bacterium and related consortium could be possibly used to improve glycerol tolerance and uptake by ruminants.

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How to Cite

VANDŽUROVÁ, A. ., BÓDY, . G. ., JAVORSKÝ, P., & PRISTAŠ, P. . (2013). Actinomyces Ruminicola G10 - The Rumen Bacterium Recovered from Glycerol Enriched Cultivation Media. Nova Biotechnologica Et Chimica, 12(1), 39–45. https://doi.org/10.2478/nbec-2013-0004

Issue

Vol. 12 No. 1 (2013)

Section

Research Articles

License

All papers published in the Nova Biotechnologica et Chimica (NBC)  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).