REPEATED-BATCH PRODUCTION OF LACCASE BY CERIPORIOPSIS SUBVERMISPORA

Authors

  • DANIELA CHMELOVÁ
  • MIROSLAV ONDREJOVIČ

DOI:

https://doi.org/10.2478/nbec-2013-0014

Keywords:

Ceriporiopsis subvermispora, laccases, repeated-batch cultivation

Abstract

The aim of this study was to set parameters of repeated-batch cultivation of Ceriporiopsis subvermispora for laccase production and evaluate the efficiency of this type of cultivation for production of selected enzyme. The suitable conditions for repeated-batch cultivation were designed on the base of study of batch cultivation of white-rot fungus C. subvermispora. C. subvermispora was cultivated in media with different concentration of casein hydrolysate as nitrogen source and glucose as carbon source. A suitable concentration of casein hydrolysate to stimulate the laccase production was 1.5 and 2.5 g/L. Laccase production was started at certain critical concentration of glucose (5 g/L). In order to improve laccase production by repeated-batch cultivation of C. subvermispora, glucose was tested in concentration 10 g/L and casein hydrolysate in concentration 1.5 g/L. During a repeated-batch cultivation was measured increase laccase activities from 177.8 to 266 U/L. It was also observed, the cultivation time needed to reach maximum laccase production was shortened to 10 days.

Downloads

How to Cite

CHMELOVÁ, D. ., & ONDREJOVIČ, M. . (2013). REPEATED-BATCH PRODUCTION OF LACCASE BY CERIPORIOPSIS SUBVERMISPORA. Nova Biotechnologica Et Chimica, 12(2), 120–128. https://doi.org/10.2478/nbec-2013-0014

Issue

Vol. 12 No. 2 (2013)

Section

Research Articles

License

All papers published in the Nova Biotechnologica et Chimica (NBC)  are published under a CC-BY licence (CC-BY 4.0). Published materials can be shared (copy and redistribute the material in any medium or format) and adapted (remix, transform, and build upon the material for any purpose, even commercially) with specifying the author(s).