The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia)

Authors

  • Jana Sedláková-Kaduková
  • Jana Kisková
  • Lenka Maliničová
  • Ivana Timková
  • Stanislav Jeleň
  • Peter Pristaš

DOI:

https://doi.org/10.2478/nbec-2019-0003

Keywords:

Autotrophic bacteria, Acidithiobacillus albertensis, Gold mine

Abstract

Sulphur-oxidising autotrophic bacterial communities in deep biosphere from weathered ore samples from active gold mine Hodruša-Hámre, Slovakia were analysed using cultivation approach followed by DNA extraction, PCR amplification and 16S rRNA gene analyses. Indirect measurement of pH changes in cultivation media evidenced the presence of acidophilic bacteria with active production of acids. The decrease of pH was observed at the beginning of isolation and later pH in range of 1.5 – 2 was maintained in both, sulphuric acid and thiosulphate, media. The presence of homogenous population of gram-negative rods was proved by Gram staining. Molecular analyses have revealed that the population of sulphur-oxidising bacteria in gold mine is dominated by a single species of Aciditiobacillus genus, identified as A. albertensis, suggesting the low level of autotrophic bacteria diversity in deep deposits. For the first time this species was isolated from weathered rocks of a gold mine subsurface environment.

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Published

2019-08-17

How to Cite

Sedláková-Kaduková, J. ., Kisková, . J. ., Maliničová, L. ., Timková, . I. ., Jeleň, S. ., & Pristaš, P. . (2019). The first evidence of Acidithiobacillus albertensis in weathered ore samples from active gold mine Hodruša-Hámre (Slovakia). Nova Biotechnologica Et Chimica, 18(1), 18–24. https://doi.org/10.2478/nbec-2019-0003

Issue

Section

Research Articles
Received 2020-02-28
Published 2019-08-17