Optimization of callus induction with enhancing production of phenolic compounds production and antioxidants activity in callus cultures of carob (Ceratonia siliqua L.)

Authors

  • Assia Lozzi Department of Plant Production, Protection and Biotechnologies. Institute of Agronomy and Veterinary Medicine Hassan II, Rabat-Instituts 6654, Morocco
  • Rachid Mentag Biotechnology Unit, Regional Center of Agricultural Research, National Institute of Agricultural Research, Rabat 415, Morocco
  • Driss Alami-Halimi Department of Plant Production, Protection and Biotechnologies, Institute of Agronomy and Veterinary Medicine Hassan II, Rabat-Instituts 6654, Morocco
  • Rabha Abdelwahd Biotechnology Unit, Regional Center of Agricultural Research, National Institute of Agricultural Research, Rabat 415, Morocco
  • Abdelhadi Abousalim Department of Plant Production, Protection and Biotechnologies, Institute of Agronomy and Veterinary Medicine Hassan II, Rabat-Instituts 6654, Morocco

DOI:

https://doi.org/10.36547/nbc.652

Keywords:

antioxidant activity, callus cultures, Ceratonia siliqua, Condensed tannin, flavonoids, polyphenols

Abstract

Carob (Ceratonia siliqua L.) is an important Mediterranean plant species with worldwide commercial and medicinal uses. The establishment of a callus culture protocol as an alternative system to produce polyphenols of chemical and pharmaceutical interest was made in the present study for the first time in carob. Explant type and the light regime are two important factors that influence morphogenic responses and biochemical production. Maximal callus induction (100 %) and biomass accumulation were obtained in cotyledon explants under both tested light regimes (16-hour photoperiod and darkness). However, leaf callus produced a higher amount of polyphenols (TPC) and flavonoids (TFC) but a lower amount of total condensed tannins (TCT) as compared to cotyledon callus. Light treatment has significantly increased TCT content but decreased the antioxidant activity in carob callus cultures. Strong and positive correlations were obtained between TPC and TFC and the antioxidant activities with correlation coefficients ranging between 0.68 and 0.98. The obtained results indicate that calli of C. siliqua have the potential for enhanced production of phenolic compounds with antioxidant activity that is favored by culture under dark condition.

Downloads

Published

2021-11-30

How to Cite

Lozzi, A., Mentag, R., Alami-Halimi, D. ., Abdelwahd, R. ., & Abousalim, A. . (2021). Optimization of callus induction with enhancing production of phenolic compounds production and antioxidants activity in callus cultures of carob (Ceratonia siliqua L.). Nova Biotechnologica Et Chimica, 20(2), e652. https://doi.org/10.36547/nbc.652

Issue

Section

Research Articles
Received 2020-08-12
Accepted 2021-08-16
Published 2021-11-30