Quantification of anti-quorum sensing and antibiofilm activity of phyllosphere bacteria against food spoilage bacteria

Abstract

Food spoilage and microbial contamination require  attention during the food production process since the presence of these bacteria can create problems including the formation of biofilms produced by these  bacteria. Biofilm formations are initiated through cell-to-cell communication which is called quorum sensing mechanism. Hence, inhibition of this communication  mechanism could be one of the solutions to inhibit  biofilm formation. Therefore, exploration of bioactive compounds from various sources including  hyllosphere bacteria with anti-quorum sensing inhibition activities is important. Phyllosphere bacteria are a community of bacteria found on the surface of plant leaves at a very  large population. These bacteria can produce bioactive compounds that can inhibit quorum sensing mechanism. In this study, 54 phyllosphere bacteria  isolates were tested, 8 bacterial isolates had potential effect to inhibit quorum sensing. From biofilm inhibition assay, the highest percentages were showed by  ifferent phyllosphere isolates against each pathogen. Whereas, for biofilm destruction assay, JB 8F isolate had the highest percentage of destruction biofilm activity  against biofilm formed by Bacillus cereus and  Shewanella putrefaciens. Eight isolates of phyllosphere  bacteria had the potential as quorum quencher and  anti-biofilm agents, both for inhibition and destruction of biofilm.