Phenotypic, genotypic, and technological characterization of new lactic acid bacterium isolated from traditional dairy product in Djelfa, Algeria

Authors

  • Guetouache Mourad 1Department of Microbiology and Biochemistry, Faculty of Science, University Mohamed Bouadiaf of M'sila, Bp 166 M’sila 28 000, Algeria, 2Department of Biology, Faculty of Sciences, University 1 Ahmed Benbella of Oran, Algeria
  • Omrane Toumatia 3Agro-Pastoralism Research Center (APRC), Djelfa, Algeria, 4Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba (ENS-Kouba), Algiers, Algeria, 5Laboratoire de Valorisation et Bio-Ingénierie des Ressources Naturelles (LVBRN), University of Algiers 1, Algiers, Algeria
  • Samir Medjekal Department of Microbiology and Biochemistry, Faculty of Science, University Mohamed Bouadiaf of M'sila, Bp 166 M’sila 28 000, Algeria
  • Bettache Guessas Department of Biology, Faculty of Sciences, University 1 Ahmed Benbella of Oran, Algeria
  • Sarah Cheriet Laboratory of Bacteriology Research, Department of Biology, Faculty of Sciences, Tunisian Institute of Veterinary Research, University el Manar-Tunis, Tunis-Tunisia
  • Noureddine Bouras 4Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba (ENS-Kouba), Algiers, Algeria, 8Department of Biology, Faculty of Natural and Life Sciences and Earth Sciences, University of Ghardaïa, Ghardaïa, Algeria

DOI:

https://doi.org/10.36547/nbc.904

Keywords:

Biological inhibition, Lactic acid bacteria, Traditional dairy products

Abstract

A total of 33 lactic acid bacteria (LAB) were isolated from 5 samples of traditional dairy product (Jben) collected from various livestock farms in Oued El Malha, Djelfa, the Ouled Naïl Range of north-central Algeria. The taxonomic study of the strain B04 using a multiphase approach based on morphology, physiology, molecular (16S rRNA) and phylogenetic analyses allowed correlating this strain to Enterococcaceae. The difference of the 16S rRNA gene sequence of the isolate toward the most closely related genus Enterococcus was more than 6 % suggesting that the strain B04 represents a new genus. The results of the evaluation of some physiological tests indicated that B04 exhibited good biological activities including acidifying, proteolytic and bacterial inhibition. The strain B04 was antagonistic toward Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, and Bacillus cereus ATCC 10876.

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Published

2022-07-15

How to Cite

Mourad, G., Toumatia, O., Medjekal, S., Guessas, B., Cheriet, S., & Bouras, N. (2022). Phenotypic, genotypic, and technological characterization of new lactic acid bacterium isolated from traditional dairy product in Djelfa, Algeria. Nova Biotechnologica Et Chimica, 21(2), e904. https://doi.org/10.36547/nbc.904

Issue

Vol. 21 No. 2 (2022)

Section

Research Articles

License

Copyright (c) 2022 Guetouache Mourad, Omrane Toumatia, Samir Medjekal, Bettache Guessas, Sarah Cheriet, Noureddine Bouras

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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